Enzyme Immunoassay (ELISA) for the determination of 5-Hydroxy-3-Indole Acetic Acid (5-HIAA) in urine. For research use only, not for use in diagnostic procedures.<br><br>
First, 5-HIAA is derived by methylation. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The methylated analyte in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system has reached equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450nm.<br><br>
Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.
- Assay Description:
- 20 min. incubation (RT) + 1 hour (RT) + 1 hour (RT) + 30 min. (RT) = 2 hours, 50 min. total incubation time
- Catalog number:
- IB89129R
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, ready to use
- design:
- Enzyme-linked immunosorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Please Inquire
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- 5-Hydroxy-3-Indole Acetic Acid (5-HIAA)
- Sample types:
- Urine
- Sample volume:
- 50 μL
- standards:
- 6 standards, ready to use
- Standard range:
- 0 / 0.5 - 50 mg/L
- storage:
- 2 - 8 °C
- sensitivity:
- 0.17 mg/L
- Species:
- Human
