Enzyme immunoassay (ELISA) for the determination of Adrenaline (Epinephrine), Noradrenaline (Norepinephrine), and Dopamine. Flexible test system for various biological types and volumes.<br><br>
Adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically.<br><br>
The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450nm.<br><br>
Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 19-24 hours, 50 min. total incubation time (see protocol for detailed procedure)
- Catalog number:
- IB89156R
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, ready to use
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Flexible test system for various biological sample types and volumes
- Sample volume:
- 1 - 750 μL (concentrations of unknowns must be multiplied by a correction factor, calculated based on sample volume - please see protocol)
- standards:
- 6 calibrators, ready to use
- Standard range:
- Adrenaline: 0 / 0.5 - 80 ng/mL; Noradrenaline: 0 / 0.2 - 32 ng/mL; Dopamine: 0 / 0.5 - 80 ng/mL.
- storage:
- 2 - 8 °C
- sensitivity:
- Adrenaline: 0.25 ng/mL x C*; Noradrenaline: 0.1 ng/mL x C*; Dopamine 0.25 ng/mL x C*. Sensitivity based on calculated correction factor.
