Amyloid Beta (1-x) Aβ

Solid phase sandwich ELISA for the determination of human Aβ (1-x) in EDTA-plasma, cerebrospinal fluids, serum, cell culture media or the extract from brain tissue. For research use only, not for use in diagnostic procedures.<br><br>

 

The first case of Alzheimer's disease was defined and reported in 1907 by the German scientist, Dr. A. Alzheimer. His studies have shown that this is the main cause of dementia in the elderly. The plaques which appear in the brains of Alzheimer's disease patients are mostly constituted by the Amyloid Beta (Amyloidβ, Aβ) protein. Amyloid Beta is a peptide which consists of 40 or 42 (43) amino acids, and reports show that this is cleaved from β- and γ- secretase from the amyloid precursor protein. APP is a trans-membrane protein consisting of 695, 751, or 770 amino acids. Reports have shown many variants of Amyloid Beta exist and are clarified into the culture supernatant from the APP cDNA transfected mouse neuroblastoma cell. Furthermore, in 1995, a dominant and differential deposition of distinct βamyloid peptide species, Amyloid Beta (N3pE), in senile plaques was found by Saido et al. This modified molecule, starting at the 3rd amino terminal residue, glutamate, was discovered to convert to pyroglutamate through intramolecular dehydration. The IBL Code No. 27713 can be measured Amyloid Beta (1-40) variants cleaved N terminal side by any cause. The IBL Code No. 27714, Human Amyloid Beta (1-40) (N) Assay Kit and IBL Code No. 27718 Human Amyloid Beta (1-40) (FL) Assay Kit are separately prepared when you want to measure Amyloid Beta (1-40) which held N terminal side completely. In addition, Human Amyloid Beta (1-40) (N) Assay Kit uses a polyclonal antibody as Labeled antibody. On the other hand, the Human Amyloid Beta (1-40) (FL) Assay Kit uses a monoclonal antibody. Moreover, in assay of Amyloid Beta (1-42) variants cleaved N terminal side, it is a Human Amyloid Beta (1-42) Assay Kit similarly. When measuring Amyloid Beta (1-42) variants cleaved by N terminal side, please use IBL Code No.27711, Human Amyloid Beta (1-42) Assay Kit. And, when measuring Amyloid Beta (1-42) which held N terminal side completely, please use IBL Code No.27712, Human Amyloid Beta(1-42) (N) Assay Kit. Furthermore, in measurement of Amyloid Beta (N3pE-42), please use IBL Code No.27716. Human Amyloid Beta (N3pE) assay Kit. Thus, it will be very useful for Alzheimer's research to assay Amyloid Beta (1-40), Amyloid Beta (1-42), and Amyloid Beta (N3pE-42), respectively. This kit can detect Amyloid Beta (1-x) variants which is cleaved at the C-terminal side by any cause, however, it can not detect variants which is smaller than Amyloid Beta (1-16). This kit uses a N-terminal specific monoclonal antibody as pre-coated antibody.<br><br>