Solid phase sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of Amyloid β Toxic Oligomer in cerebrospinal fluids. For research use only, not for use in diagnostic procedures.<br><br>
Oligomerization of Amyloidβ (Amyloid Beta) 42 is considered as an early event in Alzheimer ’s disease (AD). Irie et al. have proposed their hypothesis from the study of systematic proline scanning and solid-state NMR that Amyloid Beta conformer with a turn structure at positions 22 and 23 in aggregation of Amyloid Beta 42 is much high toxicity and it is also easily formed to become an oligomer.1) It has been considered that the toxic Amyloid Beta conformer is stable and easily formed to become a neurotoxic oligomer. The antibody (clone: 24B3) specifically detects a toxic Amyloid Beta conformer was established for development of the ELISA assay kit.2) This kit can measure selectively putative Amyloid Beta Oligomer in CSF.
- Assay Description:
- Overnight incubation (2 - 8°C) + 1 hour (2 - 8°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time
- Catalog number:
- 27709
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich enzyme-linked immunosorbent assay (ELISA)
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Aβ, Amyloid beta, A-beta.
- Protocol:
- Sample types:
- Cerebrospinal fluids
- Sample volume:
- 100 μL of properly prepared unknown / determination
- standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 3.13 - 200.0 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.27 pg/mL
- Species:
- Human
- Products related:
- The control set is available for sale on request
- Additional info:
- References:
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