Angiopoietin-like proteins (ANGPTLs) are secretory form proteins which are similar in structure to angiopoietin which is an angiogenesis factor, and 7 ANGPTLs have been identified. ANGPTL2 has been found to affect vascular cells and monocytes, and it have also been known that ANGPTL3 and ANGPTL4 play important roles in lipid metabolism and AGF (Angiopoietin-like growth factor)/ANGPTL6 plays in energy metabolism. These various biological effects of ANGPTL family are attracting attention as a new target of strategy against lifestyle-related diseases like metabolic syndrome or cancer. There is a report that high level of ANGPTL2 in blood observed in individuals who suffer adiposity, strong insulin resistance, diabetes and arteriosclerosis. This kit is a quantitative ELISA kit which can measure ANGPTL2 concentration in human serum or plasma.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (4°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27745
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of highly specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA-plasma and cell culture supernatant
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 0.05 - 3.5 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.01 ng/mL
- Species:
- Human
- Additional info: