Solid phase sandwich ELISA for the determination of Human ANGPTL3 ELISA in serum, EDTA plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
Angiopoietin-like protein 3 (ANGPTL3) is secreted by the liver and is a hormone-like protein involved in lipid metabolism and angiogenesis. ANGPTL3 is structurally similar to the angiopoietin family, which is involved in angiogenesis. It possesses a coiled-coil domain and fibrinogen-like domain, but no binding to the Tie family has been observed. ANGPTL3 has been found to be under gene expression regulation by LXR, and it has attracted interest as a new secretion factor.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (4°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27750
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA plasma and cell culture supernatant
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 0.47 - 30 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.08 ng/mL
- Species:
- Human
- Additional info:
- References:
