Solid phase sandwich ELISA for the determination of human ANGPTL4 in serum and EDTA-plasma. For research use only, not for use in diagnostic procedures.<br><br>
Angiopoetin-like 4 (ANGPTL4) is a member of the angiopoietin/ANGPTL family. Human ANGPTL4 consists of 406 amino acids. Its protein structure is common to the angiopoietins, with a signal peptide directing secretion, an N-terminal coiled-coil domain (CCD), a linker, and a C-terminal fibrinogen-like domain (FLD). Full-length ANGPTL4 is secreted by liver and adipose tissues and cleaved to generate circulating CCD and FLD fragments. ANGPTL4 plays important roles in lipid and glucose metabolism. It inhibits lipoprotein lipase (LPL) activity by breaking the dimmer molecule, and elevates plasma TG level. Meanwhile, it is also implicated in breast cancer metastasis to lung via the regulation of vascular integrity. Both of two antibodies used in this ELISA kit recognize the N-terminal portion of human ANGPTL4 respectively.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (4°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27749
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of highly specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum and EDTA-plasma
- Sample volume:
- 100 µL or properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 23.44 - 1500 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 17.53 pg/mL
- Species:
- Human
- Additional info:
- References:
