Cre is a 38 kDa recombinase protein from bacteriophage P1 which mediates intramolecular and intermolecular site specific recombination between loxP sites. The role of Cre is to resolve dimers of P1 that arise after replication in order to allow partitioning of the two P1 molecules at cell division. A loxP site (locus of X-ing over) consists of two 13 bp inverted repeats separated by an 8 bp asymmetric spacer region. One molecule of Cre binds per inverted repeat or two Cre molecules line up at one loxP site. The recombination occurs in the asymmetric spacer region. Those 8 bases are also responsible for the directionality of the site. Two loxP sequences in opposite orientation to each other invert the intervening piece of DNA, two sites in direct orientation dictate excision of the intervening DNA between the sites leaving one loxP site behind. Thus, this precise removal of DNA can be used to eliminate an endogenous gene or transgene activate a transgene. For research use only, not for use in diagnostic procedures.
- application:
- IHC, WB
- Catalog number:
- 18771
- Datasheet:
- formulation:
- Lyophilized product from 1% BSA in PBS containing 0.05% NaN3
- immunogen:
- Synthetic peptide for middle portion (MLHRRSGLPRPSDSNAV) of Cre recombinase
- MSDS:
- notes:
- For research use only, not for use in diagnostic procedures.
The datasheet for this product (see above) is intended to serve as an example only. Please refer to the datasheet provided with the antibody for precise details. - Other names:
- Please see datasheet
- Protocol:
- purification:
- Affinity Purified with synthetic peptide
- size:
- 50 µg
- specificity:
- Confirmed by western blotting using Cre Cos/TNE sup
- storage:
- Lyophilized product, 5 years at 2 - 8 °C, Solution, 2 years at -20 °C
- Host:
- Rabbit
