Enzyme immunoassay (ELISA) for the determination of IgG-Antibodies in human serum against the extracellular proteins: Alkaline Protease (AP), Elastase (Ela) and Exotoxin A (Exo) of Pseudomonas aeruginosa. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 2 hour incubation (37°C) + 2 hours (37°C) + 30 min. (RT) = 4 hour, 30 min. total incubation time.
- Catalog number:
- E15
- configuration:
- 3 x 96 well plates, 12x8 removable strips
- controls:
- 1 control serum, ready to use.
- design:
- Enzyme immunoassay (ELISA) technique.
- FDA Status:
- For research use only, not for use in diagnostic procedures.
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum or plasma.
- Sample volume:
- 100 μL of properly diluted unknown / determination.
- standards:
- 1 positive control and 1 negative control, used to calculate cut-off value.
- Standard range:
- Cut-off value calculated using positive and negative controls for interpreting results of unknowns.
- storage:
- 2 - 8 °C
- sensitivity:
- Please see protocol for assay-specific performance characteristics.
- Species:
- Human
