Phosphorylation of signal transduction molecules, Smad2 and Smad3, can be an important information for understanding of various biological functions of transforming growth factor (TGF)-β. TGF-β type I receptor and cyclin-dependent kinases phosphorylate both Smad2 and Smad3 at the C-terminals and at linker (middle) regions of them respectively. TGF-β signaling is mediated by Smad phosphoisoforms phophorylated at both the C-terminal and linker regions. This monoclonal antibody against for Smad2L/3L (T220/T179) recognizes the part of phosphorylated threonin (Thr220 of Smad2 and Thr179 of Smad3) located in linker region of Smad2/3 specifically. It can be used for western blotting, immunoprecipitation and immunohistochemistry. It is applicable for enzyme biochemical analysis of TGF-β signaling and makes it possible to visualize the intermolecular reaction of phosphorylated Smad signaling in human tissues and monitor them in real-time. Thus, analysis of TGF-β signaling with this antibody is expected to be widely applied to cancer research and fibrosis research, and to contribute to understanding the wide variety of life phenomena mediated by phosphoisoforms of Smad2/3. For research use only, not for use in diagnostic procedures.
- application:
- IHC, WB, IP
- Catalog number:
- 10385
- clone:
- 1A1
- concentration:
- Please see datasheet
- Datasheet:
- formulation:
- Lyophilized product in PBS containing 1% BSA and 0.05% NaN3
- immunogen:
- Synthetic peptide of phosphorylated Smad2L/3L (T220/T179)
- isotype:
- IgG1
- MSDS:
- notes:
- For research use only, not for use in diagnostic procedures.
The datasheet for this product (see above) is intended to serve as an example only. Please refer to the datasheet provided with the antibody for precise details. - Other names:
- Please see datasheet
- Protocol:
- size:
- 50 µg
- storage:
- Lyophilized product, 5 years at 2 - 8 °C; Solution, 2 years at -20 °C
- Host:
- Mouse
