Solid phase sandwich ELISA for the determination of Human ApoA5 in human serum and EDTA-plasma. For research use only, not for use in diagnostic procedures.<br><br>
ApoA5 is a protein with 39kDa molecular weight that was discovered by Pennacchio et al. in 2001. It has been reported that ApoA5 is produced in the liver and binds to HDL particles and TG (Triglyceride) rich lipoprotein (CM, VLDL) and exists in blood.<br><br>
In previous studies, it has been observed that TG level in serum of ApoA5 knockout mice is significantly elevated while it is significantly decreased in serum of the knock-in mice. In addition, since it has been also suggested that ApoA5 deficiency that is caused by ApoA5 gene mutation in human shows severe hypertriglyceridaemia, it has been noted as one of factors of regulating TG metabolism.<br><br>
This ELISA can specifically measure ApoA5 in human blood.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (2 ~ 8°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27191
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum and EDTA-plasma
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0.31 - 20 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.144 ng/mL
- Species:
- Human
- Additional Info:
- References:
