Solid phase sandwich ELISA for the determination of human soluble AIM/CD5L in serum and EDTA-plasma. For research use only, not for use in diagnostic procedures. <br><br>
AIM (Apoptosis Inhibitor of Macrophage) is produced by macrophages that are one of the type of immune cell, and it is a secreted form of protein that is secreted into blood. It was named as “AIM” because of the function that suppresses apoptosis by acting on macrophage. AIM level in serum increases along with obesity and it is taken up by adipose cell. AIM decomposes triglyceride by suppression of Fatty Acid Synthase (FAS). This is because it is suggested that AIM associates with various diseases, such metabolic syndrome, that originate from lifestyle diseases (arteriosclerosis and diabetes) and fatty liver. In recent studies, it has been reported and suggested that it can be a therapeutic target for acute kidney injuries and liver cancer. AIM binds to pentamer IgM in blood, but as the antibody used in this kit can detect IgM binding form, Total AIM can be quantitatively measured by this kit.
- Assay Description:
- 60 min. incubation (37°C) + 30 min. (2 - 8°C) + 30 min. (RT) = 2 hour total incubation time
- Catalog number:
- 27265
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum and plasma (EDTA)
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 7 standards, lyophilized
- Standard range:
- 0 / 0.78 - 50 ng/mL
- storage:
- 2 - 8°C
- sensitivity:
- 0.05 ng/mL
- Species:
- Human
- Additional Info: