Solid phase enzyme immunoassay (ELISA) for the separate determination of IgG and/or IgM antibodies against phospholipids in human serum. The assay employs highly purified human Prothrombin (Factor II), Thrombin, Cardiolipin, Phosphatidyl-choline, -ethanolamine, -inositol, -serine and Sphingomyelin. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time
- Catalog number:
- AE29013
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 1 negative control, ready to use
- design:
- Solid phase enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum
- Sample volume:
- 100 µL of properly diluted (1:101) unknown / determination
- standards:
- 1 cut-off calibrator, ready to use
- Standard range:
- Cut-off calibrator is used to calculate cut-off value for the interpretation of results of unknowns
- storage:
- 2 - 8 °C
- sensitivity:
- 1.0 U/mL
- Species:
- Human
