Assay Description
90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 30 min. (37°C) = 3 hours, 30 min. total incubation time.
Catalog number
BE69185
configuration
96 Determinations, 12x8 removable strips
controls
None provided.
design
Sandwich enzyme-linked immunesorbent assay (ELISA) technique.
FDA Status
For research use only, not for use in diagnostic procedures.
notes
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
Other names
Macrophage migration inhibitory factor (MIF or MMIF) also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase.
Sample types
Human serum, plasma, body fluids, tissue homogenate or cell culture supernates.
Sample volume
100 μL of properly diluted unknown / determination.
standards
8 standards, serially diluted from 1 prepared lyophilized standard.
Standard range
0 / 156.0 - 10000.0 pg/mL
storage
2 - 8 °C
sensitivity
<20 pg/mL
Species
Human
