Sandwich enzyme-linked immunesorbent assay (ELISA) for the determination of CD80 in tissue lysates or cell culture supernates. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 90 min. incubation (37°C) + 1 hour(37°C) + 30 min. (37°C) + 15 min. (37°C) = 3 hours, 15 min. total incubation time
- Catalog number:
- BE69010
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Sandwich enzyme-linked immunesorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Cluster of Differentiation 80 (also CD80 and B7-1)
- Protocol:
- Sample types:
- Tissue lysates or cell culture supernates
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 calibrators, serially diluted from 1 prepared lyophilized calibrator
- Standard range:
- 0 / 62.5 - 4000 pg/mL
- storage:
- 2 - 8°C
- sensitivity:
- <10 pg/mL
- Species:
- Mouse
