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Developing Your Own Vitamin D Application?

Feb 18th 2020

Vitamin D assayDid you know we offer all components to develop your own Vitamin D assay? Based on your application we can work together with our scientific experts at DIASource ImmunoAssays SA to select the best components from a list of proven materials:

  • One of several Monoclonal Antibodies
  • One antigen, functionalized according to your application
  • Sets of calibrators and controls
  • Displacement solution


ANTIBODIES

Polyclonal antibodies, from sheep or rabbit, have been widely used from the 90s, in RIA (Radioimmunoassays), ELISA (Enzyme-Linked Immunosorbent assays) and CLIA (Chemiluminescence Immunoassays). While polyclonal antibodies are simple and inexpensive to produce, they usually lack specificity and suffer from batch-to-batch variability. Monoclonal antibody production require high skills and is more expensive. However, a careful selection process ensures a superior specificity profile. Moreover, state of the art production techniques ensure a constant and renewable source of antibodies and all batches are identical. These monoclonal antibodies have been successfully used in commercial RIA, ELISA and POCT (Point Of Care Testing).

ANTIGENS

We have a specific collection of Vitamin D derivatives that go along with our monoclonal antibodies. Depending on your specific application, you may want to use our carboxylic acid, amine, biotin or BSA derivatives. These Vitamin D derivatives are produced by our partner's highly skilled synthetic chemists in state of the art organic chemistry facilities.

DISPLACEMENT SOLUTION

Displacing 25OH Vitamin D from its binding proteins (VDBP) is always a big challenge in Vitamin D assay development. We offer a unique displacement solution that features the All-in-One (AIO) technology. The novel and unique displacement solution displaces the 25OH Vit D from the binding proteins. The advantage of the technique is that it works at neutral pH so it can be used in the presence of the antibodies, which is not the case for the other known methods, most of them using acidic or alkaline pH.

  • Release Solution screening kits

Vitamin D metabolites, and more particularly 25OH Vitamin D, are tightly bound to the Vitamin D Binding Protein (VDBP or DBP). In order to measure 25OH Vitamin D, an assay should include a specific solution that instantly releases the molecule from its binding protein. We are now offering screening kits, including 9 or 14 different release solutions. 

Please inquire for pricing of the release solution screening sets.

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