​Galactose-deficient IgA1 (Gd-IgA1) as a Biomarker for IgA Nephropathy

Galactose-deficient IgA1 (Gd-IgA1) is attracting a lot of attention as a critical effector molecule in the pathogenesis and progression of IgA nephropathy (IgAN) in many recent studies.

It is suggested that several O-link glycans modified regions exist in the heavy chain hinge region of human IgA1 molecules and Gd-IgA1 circulates in blood stream of the patients with the pathological condition of IgAN.

The measuring system using snail (helix aspersa; HAA) lectin has been used in numerous past studies and revealed that serum levels of Gd-IgA1 in patients with IgAN is significantly elevated compared with the level of healthy subjects or patients with renal diseases other than IgAN. Thus, the importance and purpose of measuring serum Gd-IgA1 levels has been gradually recognized.

However, since the measuring system using HAA lectin is not suitable for measuring multiple and massive samples in large scale studies due to its instability of glycan-recognizing activity, development of alternative measuring system that can quantitatively measure human Gd-IgA1 in serum with stable and reliable data has been considered an essential and urgent matter. The IBL Galactose-deficient IgA1 (Gd-IgA1) ELISA kit uses a monoclonal antibody that specifically recognizes galactose-deficient hinge sequences of human Gd-IgA1. It is a lectin non-dependent measuring system that can measure Gd-IgA1 in human serum and EDTA-plasma and is also suitable for large scale studies because of its stability.

Recent publications:

• Biomarkers for IgA nephropathy on the basis of multi-hit pathogenesis. Suzuki H. Clin Exp Nephrol. 2019 Jan;23(1):26-31.PMID: 29740706

• Galactose deficient IgA1 (GD-IgA1) in skin and serum from patients with skin-limited and systemic IgA Vasculitis. Neufeld M et al. J Am Acad Dermatol. 2019 Mar 19. pii: S0190-9622(19)30443-8.PMID: 30902725

• Significance of serum galactose deficient IgA1 as a potential biomarker for IgA nephropathy: A case control study. Bagchi S et al. PLoS One. 2019 Mar 27;14(3):e0214256.PMID: 30917188

• IgA nephropathy and IgA vasculitis with nephritis have a shared feature involving galactose-deficient IgA1-oriented pathogenesis. Suzuki H et al. Kidney Int. 2018 Jan 9. pii: S0085-2538(17)30799-8.PMID: 29329643

• Galactose-Deficient IgA1-Specific Antibody Recognizes GalNAc-Modified Unique Epitope on Hinge Region of IgA1. Yamasaki K et al. Monoclon Antib Immunodiagn Immunother. 2018 Dec;37(6):252-256.PMID: 30570353

• Novel lectin-independent approach to detect galactose-deficient IgA1 in IgA nephropathy. Yasutake J et al. Nephrol Dial Transplant. 2015 Aug;30(8):1315-21.PMID: 26109484

Product information:

Galactose-deficient IgA1 (Gd-IgA1) ELISA kit - Cat# 27600

Gd-IgA1 (KM55) Anti-Human Rat IgG Monoclonal Antibody - Cat #10777