General Introduction to Amyloid Beta Aβ | Alzheimer’s Disease

"Alzheimer’s disease disrupts critical metabolic processes that keep neurons healthy. These disruptions cause nerve cells in the brain to stop working, lose connections with other nerve cells, and finally die. The destruction and death of nerve cells causes the memory failure, personality changes, problems in carrying out daily activities, and other features of the disease.The brains of people with AD have an abundance of two abnormal structures— amyloid plaques and neurofibrillary tangles—that are made of misfolded proteins. This is especially true in certain regions of the brain that are important in memory. The third main feature of AD is the loss of connections between cells. This leads to diminished cell function and cell death.

Amyloid plaques are found in the spaces between the brain’s nerve cells. They were first described by Dr. Alois Alzheimer in 1906. Plaques consist of largely insoluble deposits of an apparently toxic protein peptide, or fragment, called amyloid beta.

We now know that some people develop some plaques in their brain tissue as they age. However, the AD brain has many more plaques in particular brain regions. We still do not know whether amyloid plaques themselves cause AD or whether they are a by-product of the AD process. We do know that genetic mutations can increase production of amyloidbeta and can cause rare, inherited forms of AD " - NIH.gov

Human Amyloid Beta Detection

The accumulation of amyloid beta protein plaques in the cerebral cortex is a hallmark of Alzheimer's disease. Amyloid-beta is produced by the proteolytic cleavage of amyloid precursor protein (APP), which is mediated by the enzymes beta- and gamma-secretase. This cleavage reaction produces a number of peptides ranging from 36 to 43 amino acids in length. Our antibodies and ELISA kits detect a variety of human amyloid-beta variants and oligomers.

ELISA Kits for Detection of Human Amyloid Beta

Amyloid Beta insoluble protein is the main component of plaques found in the brains of patients with Alzheimer’s disease. however, recent research suggests that soluble, oligomeric forms of Amyloid Beta are also involved in this disease. Amyloid beta kits are solid-phase sandwich ELISAs that use two highly specific antibodies - one is precoated onto the ELISA plate and the other is HRP-conjugated.

Antibodies for Detection of Human Amyloid Beta

These products are affinity-purified IgG antibodies that recognize human amyloid beta variants cleaved at the N-terminus or C-terminus. Our human amyloid beta antibodies are raised in mice and in rabbits using a synthetic peptide. These antibodies are suitable for Western blot (WB) detection, immunohistochemical (IHC) detection, and immunoprecipitation (IP).