New Assay | Ranibizumab (Lucentis®) (mAb-based) ELISA
Posted by Brandon Savela on Mar 15th 2018
The drug Ranibizumab (trade name Lucentis®) is a recombinant humanized IgG1 kappa isotype monoclonal antibody fragment (Fab) designed for intraocular use. Ranibizumab binds to and inhibits the biologic activity of human vascular endothelial growth factor A (VEGF-A). Ranibizumab, which lacks an Fc region, has a molecular weight of approximately 48 kilodaltons. The ImmunoGuide Ranibizumab ELISA (mAb-based) kit can be efficiently used for measuring free Ranibizumab levels in aqueous humour.
This ELISA is based on a Ranibizumab-specific monoclonal antibody (catcher Ab, ImmunoGuide clone 2A1). Diluted standards and samples are incubated in the microtiter plate coated with IG-2A1 mAb. After incubation, the wells are washed. A biotinylated anti-human kappa light chain monoclonal antibody (clone ImmunoGuide 7G8(Z) is added and binds to the kappa light chain part of Ranibizumab. Following incubation, wells are washed, and the horseradish peroxidase (HRP)-conjugated streptavidin is added and binds to the biotinylated 7G8(Z) mAb. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Ranibizumab in the sample or standard. Results of samples can be determined by using the standard curve. Preincubation of Ranibizumab with recombinant human VEGF inhibited the reaction.
Ranibizumab (Lucentis®) (mAb-based) ELISA
| Catalog Number | TM09034 |
|---|---|
| Species | Human |
| Design | Enzyme immunoassay (ELISA) technique. |
| Standards | 5 standards, dilute 1:10 prior to use. |
| Controls | None provided. |
| Sample Types | Aqueous humour |
| Sample Volume | 5 µL |
| Assay Desc. | 1 hour incubation (RT) + 30 min. (RT) + 30 min. (RT) + 15 min. (RT) = 2 hour, 15 min. total incubation time. |
| Standard Range | 0 / 10 - 300 ng/mL |
| Sensitivity | 0.33 ng/mL |
| Storage | 2 - 8 °C |
| Configuration | 96 Determinations, 12x8 removable strips. |
| FDA Status | For research use only, not for use in diagnostic procedures. |
View the package insert
