New Product Release: Alpha Klotho (α-Klotho) Soluble (Mouse) ELISA
Posted by Brandon Savela on Dec 19th 2016
New product: #27601 Alpha Klotho Soluble (Mouse) ELISA
α-Klotho is a key player that integrates a multi-step regulatory system of calcium metabolism and phosphate homeostasis. α-klotho (Alpha Klotho) was identified as an extremely down-regulated gene in the genetically modified mouse of which phenotype is very similar to various symptoms of human aging. The sequences of Alpha Klotho genes have been identified in various species including humans based on the genes of mice. Alpha Klotho protein is a 130 kDa, one-transmembrane protein and its expression is confirmed in the kidneys and the parathyroid glands. In recent years, it has become clear that Alpha Klotho is an important molecule within a living organism regulating the metabolism of minerals such as calcium and phosphorus. Therefore, it is considered that early aging-like symptoms are induced by impaired mineral homeostasis caused by decreased expression of Alpha Klotho in mice. Meanwhile, it is reported that the long N-terminal extracellular domain (which comprises the major sequence portion of Alpha Klotho protein) is released free into blood by shedding. However, there are many unclear points about functions and changes in concentration of free (soluble) Alpha Klotho protein, so it has been required to develop the measurement system of Alpha Klotho. This ELISA kit can measure soluble Alpha Klotho protein in mouse urine.
Product overview:
| Product Code | 27601 |
|---|---|
| Product Name | Mouse soluble α-Klotho Assay Kit |
| Intended Use | Research reagents |
| Measuring Method | ELISA |
| Conjugate | HRP |
| Species | Mouse |
| Measuring Samples | Urine |
| Measurement Range | 78.13 ～ 5000 pg/mL |
| Primary Reaction | 60 minutes at 37 ℃ |
| Secondary Reaction | 30 minutes at 2 - 8 ℃ |
| Sensitivity | 18.06 pg/mL |
| Specificity |
Mouse soluble α-Klotho 100%
Mouse soluble α-Klotho(KL1) 100% Mouse soluble β-Klotho ≦0.1% Human soluble α-Klotho ≦0.1% Rat soluble α-Klotho N.T |
| Storage Conditions | 2 - 8 ℃ |
| Poisonous and Deleterious Substances | Not Applicable |
| Package Size | 96 Well |
Human soluble α-Klotho protein in human blood, EDTA-plasma and urine can be measured by this assay kit: #27998 Alpha Klotho (α - Klotho) Human Soluble ELISA
Related products: #27796 FGF19 ELISA, #27797 FGF21 ELISA