GPIHBP1)

Posted by Brandon Savela on Jul 6th 2017

We have released lipase related assay kits (HTGL / EL / GPIHBP1) which can be measured with serum samples.

#27180 Human Serum HTGL ELISA kit
#27263 Human EL C-Terminal ELISA kit
#27179 Human GPIHBP1 ELISA kit

Please refer to the following articles as references.
HTGL
A new enzyme-linked immunosorbent assay system for human serum hepatic triglyceride lipase. Miyashita K et al. J Lipid Res. 2017 Jun 20. pii: jlr.M075432
PMID: 28634192
GPIHBP1
Autoantibodies against GPIHBP1 as a Cause of Hypertriglyceridemia. Beigneux AP et al. N Engl J Med. 2017 Apr 27;376(17):1647-1658.
PMID: 28402248

About HTGL
HTGL (hepatic triacylglycerol lipase / hepatic triglyceride lipase) is a secreted glycoprotein known as lipolytic enzyme or also hepatic lipase (HL). HTGL has an important role in lipoprotein metabolism as a lipolytic enzyme that hydrolyzes triglycerides (TG) and phospholipids in chylomicron remnants, intermediate-density lipoproteins (IDL) and high-density lipoproteins (HDL). It has been reported that hypercholesterolemia or triglyceridemia is observed and β-very-low-density lipoproteins (VLDL), chylomicron remnants, IDL, TG-rich low-density lipoproteins (LDL) and HDL are accumulated in patients with deficiency of HTGL.

About EL
EL (Endothelial Lipase) is a metabolic enzyme of HDL. It is a phospholipase A1 molecule that has a high substrate specificity against phospholipids that exists in high density lipoprotein, HDL molecules. It promotes HDL metabolism through metabolizing HDL phospholipids. It has been considered that EL is involved with lipid metabolism and elevating the risk of arteriosclerosis. Strong expression of EL on newly formed vessels (macrophages) in arteriosclerotic lesions is observed while EL expresses in vascular endothelial cells and vascular smooth myocytes of normal blood vessels.　

About GPIHBP1
GPIHBP1 (Glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1) is an anchor protein that is modified by glycolipids and is known that it exists on a capillary endothelial cell membrane involved with metabolism of triglyceride rich (TG-rich) lipoprotein (triglyceride). GPIHBP1 transports lipoprotein lipase (LPL) that is synthesized and secreted by adipocyte or skeletal muscle cells into capillary lumen and moors on the surface of endothelial cells. It has been revealed that GPIHBP1 has a significantly important role in TG-rich lipoprotein metabolism as it has been considered that gene mutation of GPIHBP1 is a cause of type 1 hyperlipidemia (high chylomicronemia).