Product Spotlight | α-Synuclein ELISA
Posted by Brandon Savela on Nov 28th 2017
α-Synuclein is a 14.5kDa, soluble form protein which consists of 140 amino-acid residue and it is known as an intrinsically disordered protein. It is mainly expressed in cerebral neocortex, hippocampus, nigra, thalamus and metepencephalon. The majority of it exists in the presynaptic terminal and nucleus of neuron cells. It is involved with the signal regulatory system of the synaptic vesicle. α-Synuclein is a main structural component of Lewy bodies, abnormal aggregates of protein that develop inside nerve cells in neurodegenerative diseases such as Parkinson's disease, dementia, multiple system atrophy and other disorders. It is considered that excessive accumulation of α-Synuclein is a main cause of synucleinopathy due to inducing the shedding of dopamine-producing cells.
The concentration of α-Synuclein in human blood or human CSF can be measured by our ELISA kit. This kit is a solid phase sandwich ELISA using 2 kinds of highly specific antibodies. Tetra Methyl Benzidine (TMB) is used as a coloring agent (Chromogen). The strength of coloring is proportional to the quantities of Human α-Synuclein.
For research use only, not for use in diagnostic procedures.
| Catalog Number | 27740 |
|---|---|
| Species | Human |
| Design | Solid phase sandwich ELISA using 2 kinds of highly specific antibodies. |
| Standards | 8 standards, serially diluted from 1 prepared lyophilized standard. |
| Controls | None provided. |
| Sample Types | Serum, EDTA plasma, CSF, and cell culture supernatant. |
| Sample Volume | 100 μL of properly diluted unknown / determination. |
| Assay Desc. | Overnight incubation (2-8°C) + 1 hour (2-8°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time. |
| Standard Range | 0 / 0.16 - 10.00 ng/mL |
| Sensitivity | 0.03 ng/mL |
More information: α-Synuclein ELISA