Reliable Assays for Measurement of GLP-1

Obtaining a reliable result of incretin measurement is very important for diabetes research and drug development. One of the key incretins is the intestinal peptide, glucagon-like peptide-1 (GLP-1). GLP-1 is secreted in response to nutrient intake and plays an essential role in the regulation of glucose homeostasis. There are multiple forms of GLP-1, with GLP-1(7-37) and GLP-1(7-36)NH2 considered to be biologically active. Accurate measurement of both forms of active GLP-1 is not only important for understanding the physiological mechanisms of blood glucose regulation in healthy people and type II diabetes subjects, but also for both clinical and preclinical studies of GLP-1 agonists and DPP4 inhibitors.

GLP-1, active form: #27700 GLP-1, Active form (High sensitivity) Assay Kit has now been released. This kit specifically measures GLP-1, active form (7-36 amide)(7-37) in human, mouse and rat. Since GLP-1, active form in blood samples has a very low concentration in fasted subjects, the development of a highly sensitive kit for measuring GLP-1, active form has been urged by many researchers.

The sensitivity of this kit is 0.09 pmol/L and is approximately five times higher than our current available kit. Most immunoassays available still require an extraction step of active GLP-1, which can be complicated and time-consuming. To solve this issue, our reliable and user-friendly assay kit does not require an extraction step.

Researchers have also desired a new kit that measures GLP-1, active form using a small sample size due to the difficulty in obtaining blood samples from mice and rats. We are confident to recommend our ELISA kit, as only 20μL of sample is necessary to effectively run the kit.

Key Advantages of #27700 GLP-1, Active form (High sensitivity) Assay Kit

• Low concentration can be measured
• Only a small amount of sample is required
• Highly sensitive and specific
• No extraction step required

GLP-1 (9-36/37): GLP-1 (7-36) amide and GLP-1 (7-37) are the active forms and these peptides are dissolved in blood by DPP4 (dipeptidyl peptidase-4) into GLP-1 (9-36) amide and GLP-1 (9-37) and these peptides lose their activity in a short period.

Our #27788 GLP-1 (9-36/37) ELISA kit measures inactive forms of GLP-1 such as GLP-1 (9-36) amide and GLP-1 (9-37) in human, mouse and rat samples. It can also measure total GLP-1 in EDTA-plasma which does not contain DPP4 inhibitor, since active forms of GLP-1 in blood are rapidly transformed to inactive forms in 2 to 5 minutes in a half period.

GLP-1 (9-36/37) has been heavily researched in diabetes studies, but other recent studies indicate emerging cardiovascular properties as well. One suggests that GLP-1 (9–36) amide confers selective protection against post-myocardial infarction remodeling (1). Our #27788 GLP-1 (9-36/37) kit may have potential to give more insight on the cardioprotective properties of GLP-1 (9-36/37) and expand our knowledge of GLP-1 biology (2).

Related metabolic syndrome assays

References:

1. Metabolically-inactive glucagon-like peptide-1(9-36) amide confers selective protective actions against post-myocardial infarction remodelling. Robinson E et al., Cardiovascular Diabetology, 2016 Apr 14;15:65.

2. A dual-monoclonal, sandwich immunoassay specific for glucagon like peptide-19-36/7 (GLP-19-36/7). Osborne RI et al., Clin Biochem. 2016 Aug;49(12):897-902. PMID: 27208557