Enzyme linked immunosorbent assay (ELISA) for the determination of human IgG antibodies in serum or plasma directed against Bordetella pertussis toxin. For research use only, not for use in diagnostic procedures.<br><br>
Bordetella pertussis belongs to the genus of Bordetella. It is a small coccoid, gram negative bacillus, which occurs world-wide.<br><br>
The attachment of B. pertussis and B. parapertussis to the ciliated cells in the mucosa of the human respiratory tract is mediated by adhesins. An important adhesion protein and an equally important immunogen is the so-called filamentous haemagglutinin (FHA). Colonization of the respiratory tract and establishment of infection are facilitated by the synergistic action of several virulence factors. An important virulence factor is pertussis toxin (PT) which mediates multiple biological effects such as leukocytosis, lymphocytosis, mitogenity, and increased sensitivity to histamine.
- Assay Description:
- 1 hour (37°C) + 30 min. (37°C) + 30 min. (37°C) = 2 hours total incubation time.
- Catalog number:
- IB05014
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 1 negative control, ready to use. Optional Positive Control (IB05014CON), sold separately.
- design:
- Enzyme linked immunosorbent assay (ELISA) technique.
- FDA Status:
- For research use only, not for use in diagnostic procedures.
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum or plasma (EDTA, citrate, heparin).
- Sample volume:
- 100 µL of properly diluted (1:101) unknown / determination.
- standards:
- 1 cut-off standard, ready to use.
- Standard range:
- A calculated cut-off value is used to interpret results of unknowns.
- storage:
- 2 - 8°C
- sensitivity:
- > 99%
- Species:
- Human
- Products related:
