Solid phase sandwich ELISA for the determination mouse soluble c-MPL/TPOR in serum and cell culture supernatant. For research use only, not for use in diagnostic procedures. <br><br>
Thrombopoietin promotes the growth and differentiation (proliferation) of megakaryocytes which produce platelets. TPOR (Thrombopoietin receptor), which is also called c-MPL (Myeloproliferative leukemia protein) or CD110, is a receptor for thrombopoietin. And it is suggested that TPOR may also play a role in the maintenance of hematopoietic stem cells, which are stem cells located within the bone marrow that have the potential to develop into red blood cells, white blood cells, megakaryocytes and platelets. This receptor is activated when thrombopoietin protein binds to it, and the activated receptor stimulates a signaling pathway called the JAK/STAT pathway, which transmits signals from outside the cell to the cell's nucleus and is important for controlling the production of blood cells. This ELISA kit can measure concentration of mouse soluble c-MPL/TPOR in serum or cell culture supernatant.
- Assay Description:
- 60 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27174
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None included
- design:
- Solid phase sandwich ELISA using 2 kinds of highly specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- TPOR (Thrombopoietin receptor), also called c-MPL (Myeloproliferative leukemia protein) or CD110
- Protocol:
- Sample types:
- Serum and cell culture supernatant
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 8 standards serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 21.88 - 1400.0 pg/mL
- storage:
- 2 - 8°C
- sensitivity:
- 3.5 pg/mL
- Species:
- Mouse
