Solid phase sandwich ELISA for the determination of Mouse CCL8/MCP-2 in serum, EDTA plasma and Heparin plasma. For research use only, not for use in diagnostic procedures.<br><br>
Chemokine (C-C motif) ligand8/Monocyte chemoattractant protein-2 (CCL8/MCP-2) consists of 97 amino acids and is constitutionally-classified as C-C subfamily. CCL8/MCP-2 is produced by monocytes, fibroblasts and epithelial cells, and it shows chemotactic activity to CD4+ T cells, CD8+ T cells, monocytes and NK cells. Though the biological activity of CCL8/MCP-2 in mice is still unknown, it is recently reported that CCL8/MCP-2 in plasma increases with incidence of GVHD (Graft-Versus-Host-Disease) which is a complication of bone-marrow transplantation and the study suggests that CCL8/MCP-2 is associated with immune response within a living organism of mouse.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (4°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27773
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA plasma and Heparin plasma
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 78.13 - 5000 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 49.9 pg/mL
- Species:
- Mouse
- References:
