Solid phase sandwich ELISA for the determination of Human CD134/OX40 in serum, EDTA plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
CD134/OX40 is a receptor of OX40 ligand (OX40L) transactivated by Tax of HTLV-1. OX40 is expressed in activated CD4+ and CD8+ T cells. OX40 in activated T cells has a role in proliferation of T cells and production of cytokines by binding with OX40L expressed on antigen presenting cells like dendritic cells or activated B cells. In addition, it also can be involved in activated T cell invasion to inflamed areas through binding with OX40L.
- Assay Description:
- 2 hour incubation (RT) + 1 hour (RT) + 30 min. (RT) = 3 hours, 30 min. total incubation time
- Catalog number:
- 27110
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA (Enzyme-linked Immunosorbent Assay)
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA plasma and cell culture supernatant
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 7 standards
- Standard range:
- 0 / 15.6 - 1000 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 6.3 pg/mL
- Species:
- Human
