Solid phase enzyme immunoassay for the combined determination of IgA and IgG antibodies against neo-epitopes of tissue transglutaminase (tTG) in human serum. The assay employing human recombinant tTG crosslinked with gliadin-specific peptides displays neo-epitopes of tTg which ensures a significantly increased sensitivity and specificity of the test. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time
- Catalog number:
- AE29019
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 1 positive control and 1 negative control, ready to use
- design:
- Solid phase enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- IgA and IgG antibodies against neo-epitopes of tissue transglutaminase (tTG)
- Protocol:
- Sample types:
- Human serum
- Sample volume:
- 100 µL of properly diluted (1:101) unknown / determination
- standards:
- 6 calibrators, ready to use. Alternatively, a cut-off calibrator, ready to use, may be used for qualitative determination.
- Standard range:
- 0 / 3 - 300 U/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 1.0 U/mL
- Species:
- Human
