Claudin is a transmembrane protein that 4 times goes through the membrane to be formed a gene family and a tight junction molecule that is involved in cell to cell adhesion of epithelial and endothelial cell sheets. It also gets together among several different and/or same type of Claudin. The structure consists of 2 loops that are found in extracellular and both its N-terminal and C-terminal domain in intracellular.
Fundamental structures among its family are common and its molecular weight is around 20 - 27kDa. Its C-terminal domain binds PDZ domain of ZO-1, ZO-2 and ZO-3 (protein behind the transmenbrane).
It has been known that Claudin-1, 2, 6, 7 and 15 are distributed in mouse liver and kidneys while Claucin-5 is in vascular endothelium. For research use only, not for use in diagnostic procedures.
- application:
- WB, IHC, ICC
- Catalog number:
- 18885
- Datasheet:
- formulation:
- Lyophilized product from 1% BSA in PBS containing 0.05% NaN3
- immunogen:
- Synthetic peptide of part of C terminal of Mouse Claudin-8 (SFHAEKRSPSIYSKSQY)
- MSDS:
- notes:
- For research use only, not for use in diagnostic procedures.
The datasheet for this product (see above) is intended to serve as an example only. Please refer to the datasheet provided with the antibody for precise details. - Protocol:
- purification:
- Purified with antigen peptide
- size:
- 100 µg
- specificity:
- Claudin-8 specific. Non-cross-reacts with Claudin-1, -2, -3, -4, -5, -6, -7, -12 and -15. (This confirmed by western blotting using each transfectants).
- storage:
- Lyophilized product, 5 years at 2 - 8 °C, Solution, 2 years at -20 °C
- Species:
- Mouse
- Host:
- Rabbit
