The Wieslab® Complement Factor P Quantitative Assay (COMPL FPQ) is an enzyme immunoassay (ELISA) for the quantitation of the amount of human Factor P in test samples. For research use only, not for use in diagnostic procedures.<br><br>
The complement system plays an essential role in chronic, autoimmune and infectious disease. There are three pathways of complement activation, namely the classical, the alternative and the lectin pathway. Impaired complement activity causes humans to become susceptible to repetitive fulminant or severe infections and may contribute to development of autoimmune disease. Inappropriate activation of complement contributes to chronic inflammation and tissue injury.<br><br>
In vitro activation of the complement sequence leads to the consumption of complement components which, in turn, leads to a decrease in their concentration. Thus, the determination of complement proteins or complement activity is used to indicate whether the complement system has been activated by an immunologic and/or pathogenic mechanism. Both functional and immunochemical complement measurements are used to evaluate subjects when a complement-activating disease is suspected or an inherited deficiency is possible. The level of complement activity evaluated by functional assays such as Wieslab® Complement kit takes into account the rate of synthesis, degradation, and consumption of the components and provides a measure of the integrity of the pathways as opposed to immunochemical methods which specifically measure the concentration of various complement components. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 60 min. (RT) + 30 min. (RT) + 30 min. (RT) = 2 hours incubation time
- Catalog number:
- COMPL FPQ RUO
- configuration:
- One frame with blue colored break-apart wells (12x8) sealed in a foil pack with a desiccation sachet. The wells have been coated with human IgM
- controls:
- 1 low control, ready to use.
1 high control, ready to use - design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Please Inquire
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum or EDTA plasma
- Sample volume:
- 100 µL of properly diluted (1:200) unknown / determination
- standards:
- 6 Standards diluted from stock material
- Standard range:
- 0 / 12.5 - 200 ng/mL
- storage:
- The reagents should be stored at 2 - 8° C except the positive control and activity control. The positive control and activity control should be stored at -20°C
- Species:
- Human
- Additional info:
