The Wieslab® Complement system Screen kit is an enzyme immunoassay for the determination of functional classical, MBL and alternative complement pathways in human serum. For research use only, not for use in diagnostic procedures.<br><br>
The complement system plays an essential role in chronic, autoimmune and infectious disease. There are three pathways of complement activation, namely the classical, the alternative and the recently discovered MBL pathway. Impaired complement activity causes humans to become susceptible to repetitive fulminant or severe infections and may contribute to development of autoimmune disease. Inappropriate activation of complement contributes to chronic inflammation and tissue injury. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (RT) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- COMPL 300 RUO
- configuration:
- One frame with break apart wells (4x8x3). 4 blue coloured strips for classical pathway (CP), coated with human IgM. 4 green coloured strips for MBL pathway (MP), coated with mannan. 4 red coloured strips for alternative pathway (AP), coated with LPS.
- controls:
- 1 negative control (ready to use) and 1 positive control (lyophilized) included
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Please inquire
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum
- Sample volume:
- 100 µL of properly diluted serum / determination (please see protocol, section labelled "Dilution of Serum")
- standards:
- The negative and positive controls can be used in such a way to calculate complement activity
- Standard range:
- The negative and positive controls can be used in such a way to calculate complement activity
- storage:
- The reagents should be stored at 2 - 8° C except the positive control. The positive control should be stored at -20° C
- Species:
- Human
- Additional info:
- Whitepaper:
