Enzyme immunoassay (ELISA) for the determination of human IgA antibodies directed against Coxiella burnetii in phase 1 in serum or plasma (EDTA, citrate, heparin). For research use only, not for use in diagnostic procedures.<br><br>
The Rickettsia Coxiella burnetii, a worldwide distributed pathogen, is responsible for the disease known as Q fever. The small gram-negative, obligate intracellular bacterium reproduces in the digestive system of ticks (Dermacentor marginatus) and placental trophoblasts. As a result tick faeces and afterbirths of infected mammals (particularly sheep) are highly infectious.<br><br>
ELISA test-systems are especially suited for the differential analysis of immunoglobulin classes directed against the virus.
- Assay Description:
- 1 hour (37°C) + 30 min. (37°C) + 30 min. (37°C) = 2 hours total incubation time.
- Catalog number:
- IB05035
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 1 negative and 1 positive Control, ready to use.
- design:
- Enzyme linked immunosorbent assay (ELISA) technique.
- FDA Status:
- For research use only, not for use in diagnostic procedures.
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum or plasma (EDTA, citrate, heparin).
- Sample volume:
- 100 μL of properly diluted (1:101) unknown / determination.
- standards:
- 1 cut-off standard, ready to use.
- Standard range:
- A calculated cut-off value is used to interpret results of unknowns.
- storage:
- 2 - 8°C
- sensitivity:
- > 94%
- Species:
- Human
