Solid phase sandwich ELISA for the determination of human Endothelial Lipase (EL) Full-Length in serum, EDTA-plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
Endothelial Lipase (EL) is a metabolic enzyme of HDL. It is phospholipase A1 molecule that is high substrate specificity against to phospholipid that exists in high density lipoprotein, HDL molecule and it promotes HDL metabolism through metabolizing HDL phospholipid. It has been reported that both full length EL (active form) and cleaved EL (inactive forms, 40K fragment cleaved at N terminal and 28K fragment cleaved at C terminal cleaved by proprotein convertase) exist in blood. This ELISA kit is designed by using two antibodies that detects N-terminal fragment and C terminal fragment so that it can detect full length EL in serum and EDTA-plasma with or without administration of heparin injection that is conventionally required for the measurement.<br><br>
- Assay Description:
- Overnight (2 - 8°C) + 30 min. (2 - 8°C) + 30 min. (RT) = Overnight and 1 hour total incubation time
- Catalog number:
- 27182
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Protocol:
- Sample Types:
- Human Serum, EDTA Plasma, or Cell culture supernatant
- Sample Volume:
- 100 µL of properly diluted samples
- Standards:
- 7 standards, prepared from stock material
- Standard Range:
- 0 / 15.6 - 2000 pg/mL
- storage:
- 2 - 8 °C
- Sensitivity:
- 5.7 pg/mL
- Species:
- Human
- Products Related:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Additional info:
- References:
