Solid phase sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of Endothelin-1 in serum, EDTA-plasma, tissue extracted, and cell culture supernatant. For research use only, not for use in diagnostic procedures. <br><br>
Endothelins (ETs) are isopeptides produced by vascular endothelium having potent vasoconstriction activity. The peptides are encoded by three separate genes and processed to yield 39 residue Big Endothelin (Big ET) molecule, which are further processed to the 21 amino acid sequences termed Endothelin-1 (ET-1), Endothelin-2 (ET-2) and Endothelin-3 (ET-3). All of members of the endothelin family contain two essential disulfide bridges and size conserved amino acid residues at the C-terminus. The ETs are produced by a variety of issues in vivo, including lung, kidney, brain, pituitary and placenta.
- Assay Description:
- Overnight incubation (2-8ºC) + 30 min. (37ºC) + 30 min. (RT) = Overnight + 1 hour total incubation time
- Catalog number:
- 17165
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich enzyme-linked immunosorbent assay (ELISA)
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA-plasma, tissue extracted, and cell culture supernatant
- Sample volume:
- 100 µL of properly prepared unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0.78 - 100 pg/mL
- storage:
- -20ºC
- sensitivity:
- 0.39 pg/mL
- Species:
- Human
- References:
