Solid phase sandwich ELISA for the determination of Human Big ET-1 in serum, EDTA-plasma, supernatant of cell culture media and extract from tissue. For research use only, not for use in diagnostic procedures.<br><br>
Endothelins (ETs) are isopeptides produced by vascular endothelium having potent vasoconstriction activity. The peptides are encoded by three separate genes and processed to yield 39 residue Big Endothelin (Big ET) molecule, which are further processed to the 21 amino acid sequences termed Endothelin-1 (ET-1), Endothelin-2 (ET-2) and Endothelin-3 (ET-3). All of members of the endothelin family contain two essential disulfide bridges and size conserved amino acid residues at the C-terminus. The ETs are produced by a variety of issues in vivo, including lung, kidney, brain, pituitary and placenta.
- Assay Description:
- Overnight incubation (4ºC) + 30 min. (37ºC) + 30 min. (RT) = Overnight + 1 hour total incubation time
- Catalog number:
- 27168
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA-plasma, cell culture supernatant
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 9 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 0.78 - 100 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.30 pg/mL
- Species:
- Human
- References:
