Competitive enzyme immunoassay for the quantitative measurement of Estradiol in serum or plasma (EDTA, lithium heparin, or citrate plasma).
Estradiol (1,3,5(10)-estratriene-3,17β-diol; 17β-estradiol; E2) is a C18 steroid hormone with a phenolic A ring. This steroid hormone has a molecular weight of 272.4. It is the most potent natural Estrogen, produced mainly by the Graffian follicle of the female ovary and the placenta, and in smaller amounts by the adrenals, and the male testes. Estradiol (E2) is secreted into the blood stream where 98% of it circulates bound to sex hormone binding globulin (SHBG) and to a lesser extent to other serum proteins such as albumin. Only a small fraction circulates as free hormone or in the conjugated form.
- Assay Description:
- 90 minutes (RT) + 30 minutes (RT) = 2 hours total incubation time
- Catalog number:
- IB79103
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, ready to use
- design:
- Competitive enzyme immunoassay (ELISA) technique
- FDA Status:
- 510(k) Exempt, can be used for in-vitro diagnostics
- MSDS:
- Please inquire
- Other names:
- 1,3,5(10)-estratriene-3,17β-diol; 17β-estradiol; E2
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Human serum or plasma (EDTA, lithium heparin, or citrate plasma)
- Sample volume:
- 25 µL / determination
- standards:
- 7 calibrators, ready to use
- Standard range:
- 0 / 25 - 2000 pg/mL
- storage:
- 2 - 8 °C
- Additional info:
- This assay should not be used for patients being treated with the drug fulvestrant (Faslodex®) which cross-reacts in the assay and could lead to falsely elevated test results. See also information in the assay protocol.
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