Competitive solid phase enzyme-linked immunosorbent assay (ELISA) for the determination of estradiol in rat serum. For research use only, not for use in diagnostic procedures. <br><br>
Estradiol (E2 or 17β-estradiol) is an estrogenic hormone produced by the ovaries and in smaller amounts by the adrenal cortex and testes. It is the most potent female sexual hormone and is essential for maintaining normal female functions. During the oestrous cycle which can be divided into four phases (proestrus, estrus, metestrus, diestrus) estradiol concentrations rise gradually from metestrus to proestrus and fell to barely detectable levels in estrus. The maximum concentration is reached around mid-day of proestrus. <br><br>
Apart from its effects on sexual characteristics it has important influence on the growth and development of the brain. <br><br>
In female rodents, the determination of estradiol is a useful marker in evaluating and monitoring the state of the reproductive functions and pregnancy as well.
- Assay Description:
- 2 hour incubation w/ shaking (RT) + 60 min. w/ shaking (RT) + 30 min. (RT) = 3 hour, 30 min. total incubation time
- Catalog number:
- IB79329
- Controls:
- None provided
- Design:
- Solid phase enzyme-linked immunoassay (ELISA).
- FDA Status:
- For research use only. Not for use in diagnostic procedures.
- MSDS:
- Please Inquire
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample Types:
- Serum
- Sample Volume::
- 75 µL
- Standards:
- 6 standards, lyophilized
- Standard Range:
- 0 / 5 - 1280 pg/mL
- Storage:
- 2 - 8 °C
- Sensitivity:
- 2.5 pg/mL
- Species:
- Rat
