ImmunoGuide® Enzyme immunoassay (ELISA) test kit for the determination of free Etanercept (Enbrel®) in serum and plasma.<br><br>
The drug Etanercept (trade name Enbrel®) is a dimeric fusion protein consisting of the extracellular ligand-binding portion of the human 75 kilodalton (p75) tumor necrosis factor receptor (TNFR) linked to the Fc portion of human IgG1. Etanercept binds specifically to human tumor necrosis factor alpha (TNF-α) and blocks its interaction with cell surface TNF receptors. Serum concentration of Etanercept might be related to predict some clinical outcome during maintenance therapy. The specificity of this test system is achieved by using a monoclonal antibody named "5A1 mAb" for the coating of the microtiter plate. This antibody is specific for Etanercept only and does not cross react with other TNF-α catchers. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 60 min. incubation (RT) + 30 min. (RT) + 10 min. (RT) = 1 hour, 40 min. total incubation time
- Catalog number:
- TM09006
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Available upon request
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum and plasma
- Sample volume:
- 10 µL
- standards:
- 5 standards, ready to use
- Standard range:
- 0 / 6 - 200 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 5 ng/mL
- Species:
- Human
- Products related:
- Etanercept ELISA
Anti-Etanercept ELISA - Additional info:
