Solid phase sandwich ELISA for the determination human FGF21 in serum, EDTA plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
FGF19 subfamily composed of FGF19, FGF21 and FGF23 binds the receptors, α/βKlotho and it is characterized as a factor group which has hormonal functions. The novel system consisted of α/βKlotho and FGF19 subfamily regulates integrally metabolism of minerals, lipids, energy and amino acids. This novel regulating system involves important homeostatic mechanism for biological body such as metabolism of bile acid and cholesterol in the liver (FGF19), energy and glycolipids metabolism (FGF21) and phosphate, calcium and vitamin D metabolism in kidney (FGF23) in fasting state.<br><br>
FGF21 is secreted in the liver and it is reported that it has a lipolytic action in fatty tissues. FGF21 binds a receptor consisted of FGFR and βKlotho. FGF21 reacts in emptiness and it accelerates lipid metabolism in liver, Triacylglycerol (TG) is used as lipid acid and Ketone bodies are produced by the metabolism. As a result, it is used as an energy source. In this process, FGF21 is released and acts as a hormone-like factor. In relation to the concentration of FGF21 in blood, it is measured and reported that the correlation with many diseases like gestational diabetes mellitus, mitochondrial dysfunction in HIV infection, friedreich ataxia, coronary artery disease, carotid atherosclerosis, non-alcoholic fatty liver disease, rheumatoid arthritis, diabetic nephropathy, type2 diabetes, dyslipidemia and, obesity and metabolic syndrome. This kit can assay Human blood FGF21 density.
- Assay Description:
- Overnight incubation (4°C) + 30 min. (4°C) + 30 min. (RT) = Overnight + 1 hour total incubation time
- Catalogue number:
- 27997
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA-plasma, cell culture supernatant
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 31.3 - 2,000 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 29.4 pg/mL
- Species:
- Human
- References:
