Solid phase sandwich ELISA for the determination of human fibulin-5/DANCE in EDTA-plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures. <br><br>
DANCE was identified as a ligand of integrins expressed mainly in arteries during embryogenesis and was named developmental arteries and neural crest EGF-like /DANCE. It is also called fibulin-5 and is a member of fibulin-family. Fibulin-5/DANCE is a secreted protein of 448 amino acids and 66kDa. It contains a RGD (arginine-glycine-aspartic acid) motif in the N-terminal region and binds to integrins α5β1, αvβ3 and αvβ5. The expression of mRNA is augmented in smooth muscle cell, fibroblasts and endothelial cells of injured vascular tissues. Fibulin-5-deficient mice recapitulate human aging phenotypes, such as loose skin (cutis laxa), emphysematous lungs, and stiff arteries. It is known that destruction and degradation of elastic fibers directly cause many aging-related disorders like these. Disorganized elastic fibers were observed in the fibulin-5-deficient mice and indicating that fibulin-5/DANCE is an essential protein for elastogenesis. This product is an ELISA kit which can measure human fibulin-5/DANCE.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (4°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27121
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of highly specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- EDTA-plasma and cell culture supernatant
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 0.8 - 5.0 ng/mL
- storage:
- 2 - 8°C
- sensitivity:
- 0.01 ng/mL
- Species:
- Human
- Products related:
- The control set is available for sale on request
- Additional info:
