Enzyme immunoassay (ELISA) for the determination of human IgG antibodies directed against the lipopolysaccharide (LPS) of Francisella tularensis in serum or plasma (EDTA, citrate, heparin). For research use only, not for use in diagnostic procedures.<br><br>
Tularemia is a zoonosis caused by the Francisella tularensis bacterium and which can manifest in a variety of clinical conditions. Since the condition in animals resembles that of plague and the disease often affects hares and wild rabbits, it is also called rodent plague. Other traditional names for it are rabbit fever, lemming fever or Parinaud’s disease.<br><br>
Francisella tularensis (formerly also called Pasteurella tularensis) is a gram-negative pleomorphic bacterium. The three relevant forms of the four subspecies are difficult to differentiate. Two types can be distinguished epidemiologically, biochemically and genotypically: Francisella tularensis biovar tularensis (type A) is highly virulent and, untreated, the infection has a high mortality rate. Francisella tularensis biovar holarctica (type B) is much less virulent but can also cause severe illness.<br><br>
Various small mammals, such as hares, rabbits, mice, rats and squirrels, are natural reservoirs of Francisella tularensis. The pathogen has also been detected in soil samples and water courses and can survive there for a prolonged period depending on climatic conditions. Human infection can occur through direct skin and mucosal contact with infected animals or their excreta. Ectoparasites can also be considered as vectors. Consumption of infected and insufficiently cooked meat and inhalation of infectious dust are also potential routes of infection.<br><br>
ELISA test-systems are especially suited for the differential analysis of immunoglobulin classes directed against the virus.
- Assay Description:
- 1 hour (37°C) + 30 min. (37°C) + 30 min. (37°C) = 2 hours total incubation time
- Key Features:
- Excellent sensitivity and specificity values
Use of Lipopolysaccharid for the demonstration of antibodies against Francisella tularensis Type A and Type B - Catalog number:
- IB05058
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 1 negative control, ready to use
- design:
- Enzyme linked immunosorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum or plasma (EDTA, citrate, heparin)
- Sample volume:
- 100 µL of properly diluted (1:101) unknown / determination
- standards:
- 1 cut-off standard, ready to use
- Standard range:
- A calculated cut-off value is used to interpret results of unknowns
- storage:
- 2 - 8°C
- sensitivity:
- > 99%
- Species:
- Human
