Solid phase sandwich ELISA for the determination of Gd-IgA1 (Galactose-deficient IgA1) in human serum, EDTA-plasma, and urine. For research use only, not for use in diagnostic procedures.<br><br>
Galactose-deficient IgA1 (Gd-IgA1) attracts a lot of attentions as a critical effector molecule in the pathogenesis and progression of IgA nephropathy (IgAN) in recent studies. It has been suggested that several O-link glycans modified regions exist in the heavy chain hinge region of human IgA1 molecule andGd-IgA1 circulates in blood stream of test samples with the pathological condition of IgAN. The measuring system using snail (helix aspersa; HAA) lectin that is extracted from snail has been used in past numerous studies and it was revealed that serum levels of Gd-IgA1 in samples with IgAN is significantly elevated compared with the level of healthy subjects with renal diseases other than IgAN. Thus, the importance and purpose of measuring serum Gd-IgA1 level have been gradually recognized from such studies.<br><br>
However, since the measuring system used HAA lectin is not suitable for measuring multiple and massive samples in large scale studies due to its instability of glycan-recognizing activity, development of alternative measuring system that can quantitatively measure human Gd-IgA1 in serum with stable and reliable data has been considered as an essential and urgent matter. This IBL ELISA kit using the monoclonal antibody that specifically recognizes galactose-deficient hinge sequence of human Gd-IgA1 is a lectin non-dependent measuring system that can quantitatively measure Gd-IgA1 in human serum, which is also suitable for large scale studies because of its stability.
- Assay Description:
- 1 hour incubation (RT) + 30 min. (RT) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27600
- configuration:
- 96 Determinations, 12x8 removable strips
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- design:
- Solid phase sandwich enzyme-linked immunosorbent assay (ELISA)
- MSDS:
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Other names:
- Galactose-deficient IgA1
- Sample types:
- Human serum , EDTA-plasma, and Urine
- Sample volume:
- 50 µL of properly diluted unknown / determination
- standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 1.56 - 100.0 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.488 ng/mL
- Species:
- Human
- Additional info:
- FAQ:
- References:
