Enzyme immunoassay (ELISA) for the determination of specific IgA antibodies against Gliadin in serum and plasma. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 1 hour incubation (RT) + 30 min. (RT) + 20 min. (RT) = 1 hour, 50 min. total incubation time
- Catalog number:
- IB79293
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- Optional use of standards as controls if cut-off standard is used
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum and plasma (EDTA, heparin)
- Sample volume:
- 100 µL of properly diluted (1:101) unknown / determination
- standards:
- 4 standards diluted with PBS, ready to use
- Standard range:
- A standard curve is plotted qualitatively (U/mL) using O.D. values of standards
- storage:
- 2 - 8 °C
- sensitivity:
- 1.05 U/mL
- Species:
- Human
