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Glucagon-like Peptide-1 (GLP-1) (9-36/37)

$827.00
SKU:
27788
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Solid phase sandwich ELISA for the determination of GLP-1 (9-36/37) in human, mouse, and rat EDTA plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
GLP-1 (Glucagon Like Peptide-1) is a transcriptional product of proglucagon gene as same as glucagon and one of gastrointestinal hormone which is secreted from L cells of lower section of the small intestine. GLP-1 (7-36) amide and GLP-1 (7-37) are active form and these peptides are dissolved in blood by DDP4 (dipeptidyl peptidase-4) into GLP-1 (9-36) amide and GLP-1 (9-37) and these peptides lose its activities in short period. GLP-1 is known as incretin as same as GIP (Glucose-dependent Insulinotropic Polypeptide), it promotes secretion of insulin from β cells of the isles of Langerhans in the pancreas as glucose dependently. GLP-1 also suppresses secretion of glucagon from α cell of pancreas and concentration of glucose in blood after meal. Drug of type 2 diabetes has been developed by utilizing the functional mechanism of GLP-1 such as blood sugar control through pancreas, delayed gastric emptying and appetite suppression which contributes weight loss as extra-pancreatic effects. This ELISA kit can quantitatively measure inactive forms of GLP-1 such as GLP-1 (9-36) amide and GLP-1 (9-37) specifically. It can also quantitatively measure as total GLP-1 in EDTA-plasma which does not contains DPP4 inhibitor since active forms GLP-1 in blood are rapidly transformed to inactive forms in 2 to 5 minutes in a half period.
Assay Description:
60 min. incubation (37°C) + 60 min. (4°C) + 30 min. (RT) = 2 hr 30 min. total incubation time
Catalog number:
27788
configuration:
96 Determinations, 12x8 removable strips
controls:
None provided
design:
Solid phase sandwich ELISA
FDA Status:
For research use only, not for use in diagnostic procedures
Protocol:

Protocol

notes:
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
MSDS:

MSDS

Sample Types:
EDTA-plasma and cell culture supernatant
Sample Volume:
100 μL of properly diluted unknown / determination
Standards:
8 standards, serially diluted from 1 prepared lyophilized standard
Standard Range:
0 / 1.25 - 80 pmol/L
Sensitivity:
0.36 pmol/L
Storage:
2 - 8 °C
Species:
Human, Mouse, Rat
Additional info:

Incretin Pamphlet

Assay Design