Solid phase sandwich ELISA for the determination of GLP-1 (9-36/37) in human, mouse, and rat EDTA plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
GLP-1 (Glucagon Like Peptide-1) is a transcriptional product of proglucagon gene as same as glucagon and one of gastrointestinal hormone which is secreted from L cells of lower section of the small intestine. GLP-1 (7-36) amide and GLP-1 (7-37) are active form and these peptides are dissolved in blood by DDP4 (dipeptidyl peptidase-4) into GLP-1 (9-36) amide and GLP-1 (9-37) and these peptides lose its activities in short period. GLP-1 is known as incretin as same as GIP (Glucose-dependent Insulinotropic Polypeptide), it promotes secretion of insulin from β cells of the isles of Langerhans in the pancreas as glucose dependently. GLP-1 also suppresses secretion of glucagon from α cell of pancreas and concentration of glucose in blood after meal. Drug of type 2 diabetes has been developed by utilizing the functional mechanism of GLP-1 such as blood sugar control through pancreas, delayed gastric emptying and appetite suppression which contributes weight loss as extra-pancreatic effects. This ELISA kit can quantitatively measure inactive forms of GLP-1 such as GLP-1 (9-36) amide and GLP-1 (9-37) specifically. It can also quantitatively measure as total GLP-1 in EDTA-plasma which does not contains DPP4 inhibitor since active forms GLP-1 in blood are rapidly transformed to inactive forms in 2 to 5 minutes in a half period.
- Assay Description:
- 60 min. incubation (37°C) + 60 min. (4°C) + 30 min. (RT) = 2 hr 30 min. total incubation time
- Catalog number:
- 27788
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- MSDS:
- Sample Types:
- EDTA-plasma and cell culture supernatant
- Sample Volume:
- 100 μL of properly diluted unknown / determination
- Standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard Range:
- 0 / 1.25 - 80 pmol/L
- Sensitivity:
- 0.36 pmol/L
- Storage:
- 2 - 8 °C
- Species:
- Human, Mouse, Rat
- Additional info: