Glucose-dependent Insulinotropic Peptide (GIP)(Rat), Total (High Sensitivity)

Sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of GIP (Total, Rat) in rat EDTA-plasma. For research use only, not for use in diagnostic procedures.<br> <br>

Incretin is a group of gastrointestinal hormones that promotes insulin secretion  from the beta cells of the islets of Langerhans after eating (glucose dependent) and it plays an important role for regulate blood glucose. GIP(glucose-dependent insulinotropic peptide) is incretin as same as GLP-1. It is 42 amino-acid polypeptide and it is secreted from K cells of small intestine by lipid stimulant. Two amino-acid at N-terminal of GIP is rapidly cleaved by DPP-4 in blood and it is inactivated to GIP (3-42). DPP-4 inhibitor that inhibits DPP-4 enzyme which cleaves GIP and GLP-1 and maintain incretin activation has been widely used as useful drug of type 2 diabetes. GIP receptors express not only in beta cell of pancreas but in various organs. It has been reported that especially GIP receptors in adipocyte involve with obesity due to the function of energy deposition. In many cases of the research of glycometabolism, various items are measured at same time and the volume of collected animal samples are limited if the blood needs to be collected in series. This ELISA kit can measure total rat GIP (1-42) and (3-42) using a tiny amount of rat blood sample.