Solid phase sandwich ELISA for the determination of human total GIP in EDTA-plasma and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
Incretins are a group of gastrointestinal hormones that cause an increase in the amount of insulin released from the beta cells of the islets of Langerhans after eating and they also inhibit glucagon release from the alpha cells of the Islets of Langerhans. GIP, typical incretin like GLP-1, was isolated and sequenced from intestinal mucosa as “gastric inhibitory peptide” in 1970, and then it was renamed as “glucose-dependent insulinotropic peptide”. It has been reported that GIP receptor is expressed in cells such as beta cell of pancreas, adipocyte or osteoblastic cell, and it plays essential roles in reserving of ingested nutrients within the body in each cell, and the control of GIP signal can lead to improvement of metabolic syndrome. GIP is rapidly inactivated to GIP (3-42) from active form of GIP (1-42) by DPP-IV in blood. This ELISA kit detects both types of human GIP, active form (1-42) and inactivated form (3-42), and can measure total GIP in samples.
- Assay Description:
- 60 min. incubation (37°C) + 60 min. (4°C) + 30 min. (RT) = 2 hr. 30 min. total incubation time
- Catalogue number:
- 27203
- Configuration:
- 96 Determinations, 12x8 removable strips
- Controls:
- None provided
- Design:
- Solid phase sandwich ELISA with two high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- MSDS:
- Sample Types:
- EDTA-plasma, and cell culture supernatant
- Sample Volume:
- 100 μL of properly diluted unknown / determination
- Standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard Range:
- 0 / 1.88 - 120 pmol/L
- Sensitivity:
- 0.82 pmol/L
- Storage:
- 2 - 8 °C
- Species:
- Human
- Additional Info:
- References:
