Enzyme Immunoassay (ELISA) for the determination of L-Glutamate in urine and various biological samples.<br><br>
After extraction and derivatization Glutamate is quantitatively determined by ELISA.<br><br>
The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized analyte concentrations in the standards, controls and sampled and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450nm.<br><br>
Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standards. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 10 min. incubation (RT) + 2 hours (RT) + 10 min. (RT) + 2 hours (RT) + 30 min. (RT) + 20 min. (RT) = 5 hours, 10 min. total incubation time
- Catalog number:
- IB89151R
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, ready to use
- design:
- Enzyme Immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Please Inquire
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Urine, plasma, and serum
- Sample volume:
- 100 μL of properly prepared unknown / determination
- standards:
- 6 calibrators, ready to use
- Standard range:
- 0 / 0.6 - 60 μg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.3 μg/mL
- Species:
- Human