Solid phase sandwich ELISA for the determination of Human G-CSF supernatant of cell culture media. For research use only, not for use in diagnostic procedures. <br><br>
G-CSF is the cytokine that stimulates differentiation and propagation of neutrophil precursor cells. It is produced by fibroblast and endothelial cells and it is reported to stimulate the propagation in some tumor.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (37°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27131
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Supernatent of cell culture media
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 7.81 - 500 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 1.23 pg/mL
- Species:
- Human
- Products related:
- The control set is available for sale on request
- References:
