Solid phase sandwich ELISA for the determination of Rat GRO/CINC-3 in serum, EDTA-plasma and supernatant of cell culture media. For research use only, not for use in diagnostic procedures.<br><br>
Cytokine-induced neutrophil chemo attractant 3(CINC-3) is, also known as rat MIP-2, a member of the alpha (CXC) subfamily of chemokines, which is expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. The amino acid sequence of rat CINC-3 is 88% identical to murine MIP-2. CINC-3 is also known to be a potent chemo tactic factor for rat neutrophil both in vitro and in vivo. Nakagawa’s group at Toyama Medical and Pharmaceutical Univ. in 1994 has postulated that rat neutrophil have at least two classes of CINC receptors, a class of CINC-3 specific ones and a second common receptor shared by all CINCs.
- Assay Description:
- 1 hour incubation (37ºC) + 30 min. (4ºC) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27163
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA plasma, supernatant of cell culture media
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 4.69 - 30 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.51 pg/mL
- Species:
- Rat
- References:
