GROα/MGSA as the CXC subfamily has the high homology with IL-8 in amino acid sequences. It is reported that it also has the similar configuration in 3D structural analysis and the almost same level of leukocyte chemotaxis with IL-8. Production from activated monocyte, fibroblast, epithelial and endothelial cells have been reported. In stomach mucosa infection of helicobacter pylori, individuals who suffer gastritis and ulcer in their stomach show significantly high level of IL-8 and GROα compared to non-infectious group. The GROα is identified as a factor to promote melanoma cell propagation and is a polypeptide with 3 isoforms of α, β, and? γ. The GROα depresses the propagation of endothelial cells. As renal cancer has plenty of tumor blood vessels, it is important to identify the vascular formation factor and GROα is especially important in the formation of blood vessels.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (37°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27139
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA-plasma, supernatent of cell culture media
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 3.13 - 200 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 1.07 pg/mL
- Species:
- Human
- References:
